Entry #: 17
Date: 21 October 2017
Section: Platelet function
Topic: Vitaminized olive oil and platelets
Type: Human volunteer trial

Download PDF

OliveNetTM Journal Club

Expert review of literature related to olives and olive oil

D. Elizabeth McCord, Nancy B. Ray and Tom C. Karagiannis

Title

Effect of 1-y oral supplementation with vitaminized olive oil on platelets from healthy postmenopausal women

Author(s)

Vignini et al

Citation / Year

(1) / 2017

Keywords

Vitaminized olive oil, vitamin D3, Vitamin K1, vitamin B6, reactive oxygen species, nitric oxide

Summary

This is a single-centre human volunteer trial in which healthy postmenopausal women (n=60), were administered either vitaminized olive oli or placebo (non-vitaminized olive oil) for one year. Apart from conventional anthropometric and biochemical evaluation, the aim of this study was to investigate effects on platelet function and aggregation as well as markers of oxidative stress and membrane Na+/K+-ATPase channel activity. Briefly, participants were randomly assigned into two groups (n=30 each) and were given daily oral supplementation for one year with either 1) 20 mL per day of virgin olive oil containing phylloquinone (0.70 mg / 100 mL vitamin K1), 1,25-dihydroxy-choleciferol (50 mg / 100 mL vitamin D3), and pyroxidal 5’-phosphate (6 mg per 100 mL vitamin B6) referred to as vitaminized olive oil, or 2) non-fortified virgin olive oil which was used as a placebo. Importantly, the energy content and fat composition was similar and both the vitaminized olive oil and placebo contained 30 mg per 100 mL of natural vitamin E. Throughout the study the participants consumed a Mediterranean diet with restrictions on certain dietary supplements and food sources of vitamin K (including spinach, broccoli, asparagus, fermented foods, and certain cheeses). Postmenopausal women were chosen for this study as estrogen-deficiency is associated with increased risk for cardiovascular diseases (2). This has been, at least in part, related to loss of endothelial nitric oxide which has been shown to be reduced in postmenopausal women (3). Further, the risk for osteoporosis is increased and as the authors point out, there is a correlation between 1) nitric oxide regulation and osteoblast-osteoclast balance (4, 5) and 2) platelet function and signalling and bone mineral density (6).

Key points and implications

Firstly, the changes in anthropometric and general biochemical measurements were largely unremarkable with exception of increased high density lipoprotein (HDL)-C levels in both groups consuming olive oil for one year (T1) compared to baseline (T0). This is an interesting finding given that HDL particles are known as “good” cholesterol with protective effects against cardiovascular diseases. Regarding, oxidative stress parameters nitric oxide and reactive oxygen species were significantly decreased in women receiving oral supplementation with vitaminized olive oil for one year compared to baseline. Similarly, a decrease in membrane fluidity and an increase in Na+/K+-ATPase were observed women receiving vitaminized olive oil for one year compared to baseline. Overall, this study provides evidence for the beneficial effects of olive oil consumption and potential beneficial cardiovascular effects in postmenopausal women (HDL-C result). Further, the oxidative stress and platelet function findings with vitaminized olive oil may provide evidence for the benefits of virgin olive oil fortification.

Related publications

  1. A. Vignini et al., Effect of 1-y oral supplementation with vitaminized olive oil on platelets from healthy postmenopausal women. Nutrition 42, 92-98 (2017).
  2. T. F. Whayne, Jr., D. Mukherjee, Women, the menopause, hormone replacement therapy and coronary heart disease. Current opinion in cardiology 30, 432-438 (2015).
  3. J. Loscalzo, G. Welch, Nitric oxide and its role in the cardiovascular system. Progress in cardiovascular diseases 38, 87-104 (1995).
  4. J. Klein-Nulend, R. F. van Oers, A. D. Bakker, R. G. Bacabac, Nitric oxide signaling in mechanical adaptation of bone. Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA 25, 1427-1437 (2014).
  5. S. J. Wimalawansa, Nitric oxide and bone. Annals of the New York Academy of Sciences 1192, 391-403 (2010).
  6. A. Akbal, F. Gokmen, M. Gencer, B. S. Inceer, E. Komurcu, Mean platelet volume and platelet distribution width can be related to bone mineralization. Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA 25, 2291-2295 (2014).